ocular dorzolamide nanoliposomes for prolonged iop reduction: in-vitro and in-vivo evaluation in rabbits

نویسندگان

maryam kouchak a nanotechnology research center, ahvaz jundishapur university of medical sciences, ahvaz, iran b department of pharmaceutics, school of pharmacy, ahvaz jundishapur university of medical sciences, ahvaz, iran

reza bahmandar nanotechnology research center, ahvaz jundishapur university of medical sciences, ahvaz, iran

neda bavarsad a nanotechnology research center, ahvaz jundishapur university of medical sciences, ahvaz, iran b department of pharmaceutics, school of pharmacy, ahvaz jundishapur university of medical sciences, ahvaz, iran

fereydoun farrahi department of ophthalmology, imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, iran

چکیده

dorzolamide ophthalmic drop is one of the most common glaucoma medications but it has a short residence time in the eye. the aim of this study is to develop ocular dorzolamide hcl nanoliposomes (drz – nanoliposomes) and to evaluate their potential use for the treatment of ocular hypertension. nanoliposomes were prepared using reverse-phase evaporation vesicle (rev) and thin layer hydration (tlh) method with 7:3 and 7:4 molar ratios of phosphatidylcholine:cholesterol. the physicochemical properties of the formulations were investigated. formulations with 7:4 lipid ratio were evaluated in terms of drug release, physical stability and ex vivo permeation through the excised albino rabbit cornea. the rabbits in groups of 6 were treated with selected drz – nanoliposomes or dorzolamide solution or marketed dorzolamid preparation (biosopt®) and intraocular pressure (iop) was monitored. formulations with 7:4 molar ratio entrapped greater amount of drug compared to those with 7:3 lipid components ratio. drz – nanoliposomes with 7:4 lipid ratio showed more transcorneal permeation than dorzolamide solution (p<0.05); and the formulation prepared by tlh method exhibited higher permeability than that prepared by rev method (p<0.05). the selected drz – nanoliposomes showed greater iop lowering activity and a more prolonged effect compared to dorzolamide solution and biosopt®. drz – nanoliposomes prepared by tlh method with 7:4 ratio showed promising results as a candidate for the treatment of ocular hypertension.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ocular Dorzolamide Nanoliposomes for Prolonged IOP Reduction: in-vitro and in-vivo Evaluation in Rabbits

Dorzolamide ophthalmic drop is one of the most common glaucoma medications but it has a short residence time in the eye. The aim of this study is to develop ocular dorzolamide HCl nanoliposomes (DRZ – nanoliposomes) and to evaluate their potential use for the treatment of ocular hypertension. Nanoliposomes were prepared using Reverse-phase evaporation vesicle (REV) and thin layer hydration (TLH...

متن کامل

Ocular Dorzolamide Nanoliposomes for Prolonged IOP Reduction: in-vitro and in-vivo Evaluation in Rabbits

Dorzolamide ophthalmic drop is one of the most common glaucoma medications but it has a short residence time in the eye. The aim of this study is to develop ocular dorzolamide HCl nanoliposomes (DRZ – nanoliposomes) and to evaluate their potential use for the treatment of ocular hypertension. Nanoliposomes were prepared using Reverse-phase evaporation vesicle (REV) and thin layer hydration (TLH...

متن کامل

Ocular Dorzolamide Nanoliposomes for Prolonged IOP Reduction: in-vitroand in-vivo Evaluation in Rabbits

Dorzolamide ophthalmic drop is one of the most common glaucoma medications but it has a short residence time in the eye. The aim of this study is to develop ocular dorzolamide HCl nanoliposomes (DRZ - nanoliposomes) and to evaluate their potential use for the treatment of ocular hypertension. Nanoliposomes were prepared using Reverse-phase evaporation vesicle (REV) and thin layer hydration (TLH...

متن کامل

Preparation and in vivo evaluation of nanoliposomes containing vancomycin after intravitreal injection in albino rabbits

Objective(s): The in vivo efficacy of nanoliposomal formulation of vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) assessed.Materials and Methods: Nanoliposomal formulations were prepared and characterized. The in vivo study was carried out on rabbits which received liquid culture medium containing MRSA under anesth...

متن کامل

In-vitro and In-vivo Evaluation of Silymarin Nanoliposomes against Isolated Methicillin-resistant Staphylococcus aureus

Staphylococcus aureus is an opportunistic pathogen and remains a common cause of burn wound infections. Different studies have shown that entrapment of plant-derived compounds into liposomes could increase their anti-Staphylococcus aureus activity. Silymarin is the bioactive extract from the known plant Silybum marianum L. The objective of this study was to evaluate efficacy of silymarin in fre...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۵، شماره ۱، صفحات ۲۰۵-۲۱۲

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023